A simple, accurate, and rapid reversed phase high performance liquid chromatographic method has been developed and validated for the simultaneous estimation of Eperisone Hydrochloride (EPE) and Aceclofenac sodium (ACE) in synthetic mixture. The separation was carried out using mobile phase consisting of methanol: phosphate buffer (pH, 2.75) Water (pH, 3.0):Acetonitrile: (52:8:20:20, v/v/v). The column used was ACE 5 phenyl, (150 mm x 4.6 mm i.d., 20.0 µl) with flow rate 1.00 ml/min using PDA detection at 255 nm. The method was linear over a concentration range 2-30 μg/ml for both drugs. The retention time of Aceclofenac sodium and Eperisone hydrochloride were found to 3.2 min and 2.4 min respectively. Results of analysis were validated statistically and by recovery studies. The mean recovery was 101.19±1.50 and 99.01±0.28 for EPE and ACE respectively. The limit of detection (LOD) and the limit of quantification (LOQ) for EPE and ACE were found to be 0.36 and 1.09 µg/ml and 0.02 and 0.07 µg/ml, respectively. The results of the study showed that the proposed RP-HPLC method was found to be simple, sensitive, precise and accurate and also useful for the routine determination of EPE and ACE in mixture.
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